High-Throughput Discovery of Mutations in Tef Semi-Dwarfing Genes by Next-Generation Sequencing Analysis

Files in this data supplement:

  • Supporting Information: Figures S1-S3 and Tables S1-S6 (PDF, 4.3 MB)
  • Figure S1: 454 read distribution of 8 amplicons in the 23 superpools (PDF, 119 KB)
  • Figure S2: Sanger sequencing validation on M3 generation plants segregating for mutations in a candidate tef dwarfing gene (PDF, 439 KB)
  • Figure S3: Phenotypes of dw3 homologue mutants in M3 lines of tef (500-1: control; 893-1 and 893-9: G742A mutants; 932-12: C106T mutant) (PDF, 3.7 MB)
  • Table S1: Summary of tef genomic fosmid library (PDF, 57 KB)
  • Table S2: Primers for 454 sequencing (PDF, 83 KB)
  • Table S3: Comparison of two dw3 homoeologous genes (PDF, 58 KB)
  • Table S4: Summary of 454 reads (PDF, 54 KB)
  • Table S5: Spectrum of mutations identified in the dwarfing candidate genes (PDF, 95 KB)
  • Table S6: Comparison of expected and observed types of mutations (PDF, 53 KB)