NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Construction map of the transfer vector pNBt, used for the production of recombinant baculovirus, NeuroBactrus (Fig. S1).

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• Supplemental file 2 -

  Structure of the transfer vector pNBt and amino acid sequence of polyhedrin-Cry1-5-polyhedrin fusion protein expressed by recombinant baculovirus NeuroBactrus (Fig. S2).

  PDF, 451K

• Supplemental file 3 -

  Transmission electron microscopy of polyhedra produced by wild-type AcMNPV or NeuroBactrus (Fig. S3).

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• Supplemental file 4 -

  Fluorescence microscopy of Sf9 cells infected with wild-type AcMNPV or NeuroBactrus at 96 hours postinfection (Fig. S4).

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• Supplemental file 5 -

  Insecticidal activity of NeuroBactrus against S. exigua larvae (Fig. S5).

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• Supplemental file 6 -

  Feeding damages of NeuroBactrus-treated cabbage leafs by P. xylostella, S. litura, and S. exigua larvae (Fig. S6).

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• Supplemental file 7 -

  Schematic diagram on recovery of the NeuroBactrus to wild type (Fig. S7).

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• Supplemental file 8 -

  Northern dot blot analysis against the AaIT and cry1-5 transcripts from Sf9 cells infected with serially passed NeuroBactrus (Fig. S8).

  PDF, 64K

• Supplemental file 9 -

  Primer sequences used in quantitative real-time PCR analysis (Table S1).

  PDF, 7.5K