Detection of Putatively Thermophilic Anaerobic Methanotrophs in Diffuse Hydrothermal Vent Fluids

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    Maps showing locations of Axial Seamount and Endeavour Ridge (Fig. SA1) and of volcanoes of the Mariana Arc (Fig. SA2); dendrogram showing the phylogenetic position of the deduced amino acid sequences of mcrA genes retrieved from the FS625 sample, among other McrA sequences (Fig. SA2); description of all diffuse vent fluid samples screened with the newly designed primer pair targeting ANME-1 16S rRNA genes (Table SA1); archaeal isolates with unreported temperature optima that have a 16S rRNA gene G+C content abouve 60 mol% and are not affiliated with known thermophilic genera (Table SA2); G+C content of 16S rRNA gene sequences representing ANME microorganisms that were detected in or enriched from various hydrothermal-associated environments with indication of corresponding in situ or incubation temperatures (Table SA3.

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