Bacillus subtilis Mutants with Knockouts of the Genes Encoding Ribonucleases RNase Y and RNase J1 Are Viable, with Major Defects in Cell Morphology, Sporulation, and Competence

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Table S1, oligonucleotides

  Table S2, fold changes in sporulation- and competence-related transcripts in RNase J1 or RNase Y depletion strains compared to the wild type

  Table S3, fold changes in cell envelope biosynthesis-related transcripts in RNase J1 or RNase Y depletion strains compared to the wild type

  Fig. S1, RNase Y and J1 deletion mutants are viable in four commonly used B. subtilis genetic backgrounds

  Fig. S2, growth curves of RNase mutant strains in liquid medium

  Fig. S3, effect of temperature on RNase mutants

  Fig. S4, confirmation of double mutants by PCR

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