Supplemental Data
Files in this Data Supplement:
- Supplementary (.pdf, 397 KB) - Fig. S1 Gel-filtration chromatography of wild-type BglA-2 using Superdex 200 column. The chromogram represents the absorbance at 280 nm. The elution volume in miniliter of the peak was labeled on the top. Fig. S2 Enzymatic assays of wild-type BglA-2 and mutants towards gentiobiose-6'P (A) and maltose-6'P (B) Fig. S3 Growth profiles of wild-type, ΔBglA-2 and ΔBglA-2&ΔSP_0303 strains of S. pneumoniae TIGR4 on cellobiose as carbon source. Table S1 Kd constants of wild-type BglA-2 and mutants towards cellobiose-6'P. Table S2 Kinetic constants of wild-type BglA-2 and M423W mutant towards cellobiose-6'P or ONPβGal6P.