Files in this data supplement:
- Supporting Information: Figures S1-S7, Tables S1-S3, and Supplemental References (PDF, 1 MB)
- Figure S1: Analysis of FHA domain-Dbf4 interactions including a screen of all Y residues in Dbf4 residues 100-227 (PDF, 2 MB)
- Figure S2: Dbf4 residues V104, T105, E108, L109, and W112 were required for binding the Rad53 FHA domains. (PDF, 1 MB)
- Figure S3: Dbf4 residues V104, E108, and L109 were critical for binding the Rad53 FHA domains. (PDF, 477 KB)
- Figure S4: The synthetic lethality between dbf4-Ndelta109 and rad53-1 or rad53delta was not due to either loss of Cdc5 interaction or increased Dbf4 stability, but requires sequences between residues 82-109. (PDF, 1 MB)
- Figure S5: Evidence for a Dbf4-Dbf4 N-terminal interaction (PDF, 1 MB)
- Figure S6: Dbf4 sequences important for binding full length Rad53 and the Cdc5 PBD (PDF, 2 MB)
- Figure S7: Dbf4 T105 residue was critical for the Dbf4-FHA1 domain interaction. (PDF, 3 MB)
- Table S1: Plasmids used in this study (PDF, 123 KB)
- Table S2: Yeast strains used in this study (PDF, 119 KB)
- Table S3: Peptides used in this study (PDF, 72 KB)
- Supplemental References: PDF, 101 KB