Rational Design of a Novel Propeptide for Improving Active Production of Streptomyces griseus Trypsin in Pichia pastoris

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Strains and plasmids used in this study (Table S1), primers used in this study (Table S2), calculated parameters of the simulated structure of the trypsin mutants (Table S3), and PCR confirmation of the integrated transformants with AOX primer-5′ and AOX primer-3′ (Fig. S1).

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