Role of the Phenylalanine-Hydroxylating System in Aromatic Substance Degradation and Lipid Metabolism in the Oleaginous Fungus Mortierella alpina

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  The growth phenotypes of M. alpina grown on medium containing glucose, phenylalanine, phenylacetate, phenylalanine and p-chlorophenylalanine, tyrosine, or glucose and phenylalanine as the carbon source (Fig. S1), SDS-polyacrylamide gel electrophoresis (Fig. S2), HPLC chromatographs of reaction products (Fig. S3), a graph showing that the purified DHPR protein shows DHPR enzyme activity (Fig. S4), multiple amino acid sequence alignment of amino acid hydroxylases from fungi, animals, protists, plants, and bacteria (Fig. S5), phylogenetic tree of amino acid hydroxylases from different organisms (Fig. S6), genome sequences (Table S1), and the status of fatty acids in M. alpina and A. oryzae grown on PAH inhibitor medium (Table S2).

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