Extracellular Location of Thermobifida fusca Cutinase Expressed in Escherichia coli BL21(DE3) without Mediation of a Signal Peptide

Files in this Data Supplement:

• Supplemental file 1 -

  Oligonucleotide primers for the construction process (Table S1), effects of pH and temperature on activity and stability of cutinases (Fig. S1), and Michaelis-Menten curves of cutinases using pNPB as a substrate (Fig. S2).

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