Published August 5, 2013 // JCB vol. 202 no. 3 495-508
The Rockefeller University Press, doi: 10.1083/jcb.201302078

JIP1 regulates the directionality of APP axonal transport by coordinating kinesin and dynein motors

• Video 1  JIP1 depletion disrupts APP-YFP motility in DRGs.

• Video 2  JIP1 activates KHC-Halo motility in a single molecule TIRF assay.

• Video 3  Addition of FLAG-p150Glued-CBD inhibits JIP-mediated enhancement of KHC-Halo motility in a single molecule TIRF assay.

• Video 4  Expression of p150Glued-CBD disrupts APP-DsRed transport in DRGs.

• Video 5  Phosphorylations mutants at JIP1-S421 alter KHC activation in vitro.

• Video 6  Phosphorylation mutants at JIP1-S421 selectively enhance APP transport in one direction in DRGs.

• Supplemental material (PDF)