Engineered Biosynthesis of Disaccharide-Modified Polyene Macrolides

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  MS detection of amphotericin A (Fig. S1) and amphotericin B (Fig. S2) from S. nodosus containing pIAGO; MS detection of mannosyl amphotericin A (Fig. S3) and amphotericin B (Fig. S4) from S. nodosus containing pIAGO-nypY; HPLC analysis of heptaenes from S. nodosus KR16 containing pIAGO or pIAGO-nypY (Fig. S5); MS detection of 7-oxo-amphotericin B from S. nodosus KR16 containing pIAGO (Fig. S6); MS detection of mannosyl-7-oxo-amphotericin B from S. nodosus KR16 containing pIAGO-nypY (Fig. S7); HPLC analysis of candicidins from S. albidoflavus containing pIAGO, pIAGO-pegA1, or pIAGO-nypY (Fig. S8); MS detection of candicidins from S. albidoflavus containing pIAGO (Fig. S9); MS detection of mannosyl-candicidins from S. albidoflavus containing pIAGO-pegA1 (Fig. S10) and from S. albidoflavus containing pIAGO-nypY (Fig. S11).

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