Bacterial Community Response to Petroleum Hydrocarbon Amendments in Freshwater, Marine, and Hypersaline Water-Containing Microcosms

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    Physicochemical properties of the water samples (Table S1); identification of the bacterial isolates (Table S2); world map showing the localization of Rio de Janeiro (Brazil), the primary Brazilian offshore oil-reservoir field (Santos Basin), and the Massambaba Environmental Protection Area with sampling sites (Fig. S1); DGGE fingerprints and the dendrogram obtained after cluster analysis using the Ward and similarity Pearson coefficients to compare the structures of the hydrocarbon-enriched bacterial communities from the contaminated microcosms (Fig. S2); network analysis constructed using the Cytoscape program showing the unique and shared bacterial OTUs among the different samples (Fig. S3); unweighted and weighted UniFrac principal coordinate analysis (PCoA) ordination diagram generated based on data obtained from clone libraries (OTU = 97%) (Fig. S4).

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