Cumate-Inducible Gene Expression System for Sphingomonads and Other Alphaproteobacteria

Supplemental material

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  Supplemental Material and Methods: construction of TERM193 and pQF; oligonucleotides used in this work (Table S1); putative housekeeping genes used for MEME motif search (Table S2); genomic context, construction, and sequence of TERM193 (Fig. S1); construction of plasmid pQF (Fig. S2); alignment of the 100 base pairs upstream of the rpmB open reading frame from different sphingomonads (Fig. S3); sequence alignment of the 14 identified strong promoters (Fig. S4).

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