Isolation of Butanol- and Isobutanol-Tolerant Bacteria and Physiological Characterization of Their Butanol Tolerance

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  Primers used for the amplification, direct sequencing, and cloning of the cfa gene of strain CM4A (Table S1), cell surface hydrophobicity of strain CM4A grown with or without butanol (Table S2), changes in the membrane fatty acid compositions of E. coli strains in response to IPTG (Table S3), growth of E. coli strains in the presence of butanol or isobutanol (Fig. S1), SDS-PAGE analysis of cfa gene expression (Fig. S2), and butanol and isobutanol tolerance of strain GK12 (Fig. S3).

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