Effects of Metal Ions on Stability and Activity of Hyperthermophilic Pyrolysin and Further Stabilization of This Enzyme by Modification of a Ca²⁺-Binding Site

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Oligonucleotide primers used in this study (Table S1) and primer pairs, templates, and restriction enzyme sites used in the construction of expression plasmids (Table S2).

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