Supplemental Data
Files in this Data Supplement:
- Supplemental Figure 1 - Comparison of total ion chromatographic patterns of LC-ESI-MS/MS methodology. Typical chromatogram of the standard BA mixture (A), and the representative chromatographic patterns of the healthy control (B), early cirrhosis (C) and advanced cirrhosis (D) The analytical conditions are detailed in the experiment section.
- Supplemental Figure 2 - Comparison of total ion chromatographic patterns of GC-MS methodology. Typical chromatogram of the standard BA mixture (A), and the representative chromatographic patterns of the healthy control (B), early cirrhosis (C) and advanced cirrhosis (D) The analytical conditions are detailed in the experiment section. The chromatogram in each condition (B-D) shown in this figure was obtained from same specimen in the Figure 1 and Figure S1.
- Supplemental Figure 3 - Time-course experiment for the hydlolysis of ethyl lithocholate; Ethyl lithocholate (Ca. 0.5mg) was dissolved in 500μL of 0.1N NaOH (in 50% isopropanol), and the mixture was stand either or (A) at room temperature or (B) at 60oC . Aliquot (~1uL) of each reaction mixture was loaded on the C18 TLC plate at the time point of 15min, 30min, 1h, 2h, and 3h. TLC plate was developed with 95%methanol containing 0.5%AcOH. The spots were visualized by 50% H2SO4 followed by heating at 120oC.
- Supplemental Figure 4 - Time-course experiment for the hydlolysis of stearyl ethyl lithocholate; Ethyl lithochol-3-stearate (Ca. 0.5mg) was suspended in 500μL of 0.1N NaOH (in 50% isoPrOH), and the mixture was stand either or (A) at room temperature or (B) at 60oC . Aliquot (~1uL) of each reaction mixture was loaded on the C18 TLC plate at the time point of 15min, 30min, 1h, 2h, and 3h. TLC plate was developed with 95%methanol containing 0.5%AcOH. The spots were visualized by 50% H2SO4 with heating at 120oC.
- Supplemental Table 1 - Comparison of ethanol and aqueous NaOH on the efficiency of fecal bile acid extraction.