Assessing Genetic Diversity among Brettanomyces Yeasts by DNA Fingerprinting and Whole-Genome Sequencing

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 -

    Nonmetric multidimensional scaling plot (Fig. S1), genome nucleotide variation (Fig. S2), graphical representation (using ACT) of the presence of a β-galactosidase gene, together with the nitrate assimilation gene cluster (consisting of a nitrate reductase, nitrite reductase, and nitrate transporter gene) in AWRI 1499 and CBS 2499 (Fig. S3), maximum likelihood tree (Fig. S4), and primers used to complement/confirm bioinformatic analyses (Table S1).

    PDF, 891K

  • Supplemental file 2 -

    Single nucleotide polymorphisms (SNPs) in B. bruxellensis ST05.12/22 in comparison with the reference strain AWRI 1499 (Data Set S1).

    XLSX, 3.8M

  • Supplemental file 3 -

    Indels in B. bruxellensis ST05.12/22 in comparison with the reference strain AWRI 1499 (Data Set S2).

    XLSX, 761K

  • Supplemental file 4 -

    Single nucleotide polymorphisms (SNPs) in B. bruxellensis ST05.12/22 in comparison with the reference strain CBS 2499 (Data Set S3).

    XLSX, 4.8M

  • Supplemental file 5 -

    Indels in B. bruxellensis ST05.12/22 in comparison with the reference strain CBS 2499 (Data Set S4).

    XLSX, 239K

  • Supplemental file 6 -

    Large deletions (>1 kb) in B. bruxellensis ST05.12/22 in comparison with the reference strain AWRI 1499 as determined by read depth analysis (CNVnator) (Data Set S5).

    XLSX, 47K

  • Supplemental file 7 -

    Large deletions (>1 kb) in B. bruxellensis ST05.12/22 in comparison with the reference strain CBS 2499 as determined by read depth analysis (CNVnator) (Data Set S6).

    XLSX, 77K

  • Supplemental file 8 -

    Unique genes found in B. bruxellensis AWRI 1499 as determined by Blast analysis of the AWRI 1499 genome sequence against the ST05.12/22 assembly (Data Set S7).

    XLSX, 27K

  • Supplemental file 9 -

    Unique genes found in B. bruxellensis CBS 2499 as determined by Blast analysis of the CBS 2499 genome sequence against the ST05.12/22 assembly (Data Set S8).

    XLSX, 14K

  • Supplemental file 10 -

    Unique genes found in B. bruxellensis ST05.12/22 as determined by Blast analysis of the ST05.12/22 genome assembly against the AWRI 1499 (Data Set S9) and CBS 2499 (Data Set S10) genome sequences.

    XLSX, 21K