Transcription-Independent Functions of an RNA Polymerase II Subunit, Rpb2, During Genome Rearrangement in the Ciliate, Oxytricha trifallax

Files in this data supplement:

  • Supporting Information: Figures S1-S7 and Files S1-S2 (PDF, 1 MB)
  • Figure S1: Rpb1 localization and lncRNA expression during development in Oxytricha trifallax. (PDF, 313 KB)
  • Figure S2: Genome-wide mapping for Rpb1 ChIP at 12hrs. (PDF, 344 KB)
  • Figure S3: dsRNA localization during development in Oxytricha. (PDF, 263 KB)
  • Figure S4: Clustal-W Multiple sequence alignment of Rpb2 proteins. (PDF, 421 KB)
  • Figure S5: mRNA expression of Rpb1 paralogs throughout development. (PDF, 186 KB)
  • Figure S6: Otiwi1 staining during early sexual development in Oxytricha. (PDF, 222 KB)
  • Figure S7: Rpb2-a knockdown does not affect the levels of 27nt small RNAs. (PDF, 219 KB)
  • File S1: The Alignment identity matrix for Rpb2 multiple sequence alignment (related to Figure S4). (.xlsx, 50 KB)
  • File S2: The contig names, scores and peptide sequences recovered from Rpb2 IP LC-MS for IgG control and two Rpb2 IP replicates at 18hrs (related to Figure 3A, Table 2). (.xlsx, 341 KB)