The Key to Acetate: Metabolic Fluxes of Acetic Acid Bacteria under Cocoa Pulp Fermentation-Simulating Conditions

Supplemental material

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• Supplemental file 1 -

  List of reactions and their corresponding annotated genes (Table S1), cultivation profile of A. pasteurianus NCC 316 (Fig. S1) and A. ghanensis DSM 18895 (Fig. S2) in cocoa pulp simulation medium, mass isotopomer distributions of the carbon backbones of proteinogenic amino acids (Tables S2, S3, S4, and S6), metabolic origin of amino acids during the first growth phase of A. pasteurianus NCC 316 and A. ghanensis DSM 18895 on cocoa pulp simulation medium (Table S5), experimental mass isotopomer distributions of the carbon backbones of acetate and acetoin (Tables S7 and S8), estimated ¹³C mass isotopomer distributions of precursors derived from proteinogenic amino acids (Tables S9 and S10), input model for OpenFLUX (Table S11), experimental mass isotopomer distributions of fragments used in flux estimation (Table S12), simulated and experimental mass isotopomer distributions (Fig. S3), and equations.

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