Molecular Characterization of Three Lactobacillus delbrueckii subsp. bulgaricus Phages

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    MEME motif analysis of nucleotides upstream of start codons of all Ld25A ORFs confirm that the Shine-Dalgarno sequences follow the normal consensus sequences (Fig. S1); ClustalW alignment of 6 Lb. delbrueckii phage antireceptors: Ldb, c5, Ld3, Ld17, Ld25A (Lb. delbrueckii subsp. bulgaricus), and LL-Ku (Lb. delbrueckii subsp. lactis) (Fig. S2); RT-PCR products for Ter1, Ter2, and Ter3 (Fig. S3); confirmatory PCRs on (A) cDNA to confirm product amplification in the absence of a potential terminator sequence,(B) on total extracted RNA to ensure that products were not amplified from residual DNA after DNase treatment, and (C) on Ld17 genomic DNA to ensure primer specificity (Fig. S4).

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