Mycobacterial Gene cuvA Is Required for Optimal Nutrient Utilization and Virulence

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Supplemental methods. Table S1. Primers, plasmids, and strains used in this study.

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• Supplemental file 2 -

  Fig. S1. PCR analysis of ΔcuvA_Mt and ΔcuvA_Ms. Fig. S2. Wild-type M. tuberculosis H37Rv, ΔcuvA_Mt, and complemented ΔcuvA strains. Fig. S3. M. tuberculosis wild-type H37Rv, ΔcuvA_Mt, and ΔcuvA_Mt complemented with the wild-type cuvA_Mt allele or with alleles encoding cuvA_Mt with nonphosphorylatable or phosphomimetic substitutions grown on MM agar plus cholesterol. Fig. S4. Growth of M. smegmatis wild type, ΔcuvA_Ms, and complemented ΔcuvA_Ms strains. Fig. S5. MABA analysis of M. smegmatis wild type, ΔcuvA_Ms, and ΔcuvA_Ms complemented with cuvA_Ms, cuvA_Mt, or cuvA_Mt-gfp grown in MM plus 0.01% cholesterol. Fig. S6. Localization of PBP1-RFP. Fig. S7. Mouse infection with wild-type H37Rv, ΔcuvA_Mt, or the ΔcuvA_Mt complemented strain. Fig. S8. Relative expression of genes in the Rv1421-Rv1423 operon in wild-type H37Rv, ΔcuvA_Mt, and ΔcuvA_Mt complemented strains.

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• Supplemental file 3 -

  Movie S1. Time-lapse movie.

  AVI, 5.4M