Files in this Data Supplement:
Supplemental methods. Table S1. Primers, plasmids, and strains used in this study.
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Fig. S1. PCR analysis of ΔcuvAMt and ΔcuvAMs. Fig. S2. Wild-type M. tuberculosis H37Rv, ΔcuvAMt, and complemented ΔcuvA strains. Fig. S3. M. tuberculosis wild-type H37Rv, ΔcuvAMt, and ΔcuvAMt complemented with the wild-type cuvAMt allele or with alleles encoding cuvAMt with nonphosphorylatable or phosphomimetic substitutions grown on MM agar plus cholesterol. Fig. S4. Growth of M. smegmatis wild type, ΔcuvAMs, and complemented ΔcuvAMs strains. Fig. S5. MABA analysis of M. smegmatis wild type, ΔcuvAMs, and ΔcuvAMs complemented with cuvAMs, cuvAMt, or cuvAMt-gfp grown in MM plus 0.01% cholesterol. Fig. S6. Localization of PBP1-RFP. Fig. S7. Mouse infection with wild-type H37Rv, ΔcuvAMt, or the ΔcuvAMt complemented strain. Fig. S8. Relative expression of genes in the Rv1421-Rv1423 operon in wild-type H37Rv, ΔcuvAMt, and ΔcuvAMt complemented strains.
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Movie S1. Time-lapse movie.
AVI, 5.4M