Supplemental Data
Files in this Data Supplement:
- Fig. S1 - Growth conditions of B. longum BBMN68 in the presence of different concentrations of ox-bile: (■) 0, (◆) 0.6, (▲) 0.75, (×) 0.9, (○) 1.2 g/l.
- Fig. S2 - Comparing RNA-seq data and identifying differentially expressed genes in B. longum BBMN68. (A) Effective unique-mapping-reads distribution. (B) Scatterplot comparing the number of reads for each gene. (C) Differentially expressed genes (red points) identified by the MA-plot-based method with random sampling model at a false discovery rate of 0.001. BNCK, B. longum BBMN68 grown without bile; BNOG, B. longum BBMN68 grown in the presence of 0.75 g/l ox-bile.
- Fig. S3 - Cell surface hydrophobicity of B. longum BBMN68 was increased upon bile exposure. Values are mean ± standard deviation of one representative of two independent experiments performed in triplicate. Abbreviations: CK, BBMN68 grown without bile; OG, BBMN68 grown in the presence of bile.
- Fig. S4 - Enhanced bile survival conferred by bifidobacterial pstS on L. lactis NZ9000. Survival is calculated as the ratio of the number of colonies obtained on GM17 plates after and before different concentrations of ox-bile challenge. Data are mean ± standard deviation of three independent experiments.
- Table S1 - Oligonucleotides and primers used in this study.
- Table S3 - Transcription level of genes involved in transmembrane transport in B. longum BBMN68 in the presence of bile.
- Table S4 - Predicted target genes of response regulator RegX3 by B1H and Target Explorer.
- Table S2 - Genes were up- and down-regulated by > 3-fold in the transcriptome of B. longum BBMN68 under bile stress condition.