Files in this Data Supplement:
U immobilization and reduction by WT and mutant (pilA and pilA+) biofilms grown for 24, 48, and/or 72 h (Table S1); statistical analyses of biofilm structural parameters determined from confocal micrographs of the biofilms with the COMSTAT software (Table S2); EXAFS modeling results for R and σ2 (Table S3); EXAFS modeling results for coordination numbers (Table S4); elemental analyses of biofilm-associated precipitates (Fig. S1); CLSM micrographs showing top view projections of 24-, 48-, and 72-h WT biofilms and 48-h pilA and pilA+ biofilms stained with the BacLight viability kit (Fig. S2); U immobilization and reduction per biofilm biomass unit (Fig. S3); correlation between the age of the WT, pilA, and pilA+ biofilms and biofilm structural parameters such as biomass, thickness, surface area, surface coverage, surface-to-volume ratio, and roughness coefficient (Fig. S4); linear correlation between the total protein content of biofilms and biofilm age in plastic wells of 6-well plate and coverslip assembly assays (Fig. S5); correlation between U reduction by 48-h-old pilA, WT, and pilA+ biofilms and the biomass volume or piliation (Fig. S6).
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