Development of a Reverse Genetic System for Infectious Salmon Anemia Virus: Rescue of Recombinant Fluorescent Virus by Using Salmon Internal Transcribed Spacer Region 1 as a Novel Promoter

Supplemental material

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  Sequence alignments of the 3′ and 5′ UTR of segment 6 of different isolates of ISAV (Fig. S1), RMSDs of HE-EGFP-insert1 and HE-WT embedded in POPC membrane (Fig. S2), cytopathic effect of passage 4 of rISAV^S6/EGFP-HPR in ASK cells (Fig. S3), and colocalization of EGFP and HE proteins in ASK cells infected with rISAV^S6/EGFP-HPR (Fig. S4).

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