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Red soil sampling locations in this study (Table S1), compositions of media used in this study for isolation and fermentation of red soil actinomycetes (Table S2), distribution of the sequenced isolates at family and genus levels in different sampling locations (Table S3), OTUs and isolates that share <99% 16S rRNA gene sequence similarity with the nearest type strain (Table S4), antimicrobial activities detected in representatives from different families and genera (Table S5), information for the 107 selected isolates for secondary metabolite analysis (Table S6), known secondary metabolites from the selected isolates (Table S7), maximum-likelihood trees based on 16S rRNA gene sequences, showing the phylogenetic positions of 13 “ambiguous” Streptomyces OTUs sharing >99% 16S rRNA gene sequence similarity with the nearest type strain (Fig. S1), and chemical data and detailed structure elucidation of the novel secondary metabolites (Appendix S1).
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