Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie 2 - Video2. Wash is required for the third developmental migration in hemocytes. Time course projections of GFP expressing hemocytes undergoing developmental migrations along the ventral surface of stage 12 embryos. control(Pxn-Gal4) (A), washRNAi(2)(B), andwashRNAi(3)(C) hemocytes were imaged once per minute for 3 hours. Scale bar = 40 μm.
- Movie 3 - Video3.The Rho1 KDQ substitution mutation, but not the adjacent F39V point mutation or α-cateninRNAi, is required for the third developmental migration in hemocytes. Time course projections of GFP expressing hemocytes undergoing developmental migrations along the ventral surface of stage 12 embryos. Rho11B P{Rho1WT} (A), Rho11BP{Rho1KDQ/AAA} (B), Rho11BP{Rho1F39V} (C), and α-cateninRNAi(2)(D) hemocytes were imaged once per minute for 3 hours. Scale bar = 40 μm.
- Movie 4 - Video4.Arp2/3, but not WASH complex components, is required for the third developmental migration in hemocytes. Time course projections of GFP expressing hemocytes undergoing developmental migrations along the ventral surface of stage 12 embryos. SWIPRNAi(A), StrumpellinRNAi(B), CCDC53RNAi(C), andArp3RNAi (D)hemocytes were imaged once per minute for 3 hours. Scale bar = 40 μm.
- Movie 1 - Video1. Rho1 is required for the third developmental migration in hemocytes. Time course projections of GFP expressing hemocytes undergoing developmental migrations along the ventral surface of stage 12 embryos. control (Pxn-Gal4) (A), Rho11B(B), and Rho1RNAi(C) hemocytes were imaged once per minute for 3 hours. Scale bar = 40 μm.