Fig. S1. Disruption of the pbhmgb2 gene. Fig. S2. Kaplan-Meier survival plots of C57BL/6 mice infected with either PbANKA WT (ECM reference) or Δhmgb2 PbANKA clones. Fig. S3. Determination of the number of polymorphonuclear neutrophils and macrophages in the brains and spleens of mice infected with WT or Δhmgb2 PbANKA parasites. Fig. S4. Multiple alignment of HMGB domains from eukaryotic and Plasmodium proteins, and comparison of the TNF-α activating domains of P. falciparum and P. berghei with the murine domain of box A and box B sequences of both HMGB1 and HMGB2 proteins. Table S1. Oligonucleotides used for PCR of wild-type and recombinant parasites. Table S2. Oligonucleotides used for the transcript analyses by RT-qPCR.
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