Fig. S1. aspK and cafA deletion mutants show similar levels of TcpA as the wt under AKI conditions. Fig. S2. Growth curves of V. cholerae strains grown under AKI induction conditions. Fig. S3. Heterogeneous expression of tcpA in wt V. cholerae is seen during infection of suckling mice. Fig. S4. GFP expression from chromosomal PctxAB-gfp in the indicated strains after growth under AKI conditions, as determined by flow cytometry. Fig. S5. Flow cytometry assay of TcpA expression after the indicated strains were grown under AKI conditions. Fig. S6. Growth curves of the wt and PTS mutants incubated in LB. Table S1. Primers used for this study. Table S2. Genes found by TIS to be required for expression and Carb resistance from PtcpA-bla.
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