Analysis of Polygenic Mutants Suggests a Role for Mediator in Regulating Transcriptional Activation Distance in Saccharomyces cerevisiae

Files in this data supplement:

  • Supporting Information: File S1, Tables S1-S7, and Figures S1-S2 (PDF, 955 KB)
  • File S1: PERL script to calculate the frequency of all SNPs in two matched pools. (PDF, 477 KB)
  • Table S1: S. cerevisiae strains. (PDF, 71 KB)
  • Table S2: Oligonucleotides. (PDF, 64 KB)
  • Table S3: Crosses for bulk segregant analysis. (PDF, 35 KB)
  • Table S4: Sequencing coverage of segregant pools. (PDF, 37 KB)
  • Table S5: Causal mutant candidates. (PDF, 39 KB)
  • Table S6: Number of genes with a two-fold or greater change in RNA levels in the polygenic mutants. (PDF, 41 KB)
  • Table S7: Adjacent gene pairs enrichment. (PDF, 68 KB)
  • Figure S1: Evidence for disomy in two of the polygenic mutants. (PDF, 591 KB)
  • Figure S2: Western analysis of Med3. (PDF, 550 KB)