Fig. S1. (A) Parasite burden was measured at day 14 p.i. in the spleen, liver, and gut from mice infected per orally or i.p. with 2 x 107 spores. (B) The E. cuniculi serotiter was measured in the sera of these animals at the same time point. (C) The parasite burden in the stool of each animal was quantified every other day until day 14 p.i. Fig. S2. (A) The number of MHC class II+ CD11c+ DC in the spleen of C57BL/6 mice was measured at different time points p.i. (B) The number of MHC class II+ CD11c+ DC in the spleen of animals treated with FTY720 was assessed at day 2 p.i. (C) The histogram shows MHC class II expression by splenic CD11c+ cells at day 2 p.i. in mice treated or not with FTY720, while the graph represents MFI for MHC class II. Fig. S3. (A) RALDH activity (measured by Aldefluor fluorescence) and IL-12 expression (YFP) in the CD11b− CD8+ DC subset was compared in LP and MLN of IL-12p40 YFP mice at day 2 p.i. Graphs represent frequency (B) and total number (C) of IL-12+ Aldefluor-positive cells in MLN (top) and Aldefluor-positive cells only in the LP (bottom).
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