Uncovering Cryptic Asexuality in Daphnia magna by RAD Sequencing

Files in this data supplement:

  • Supporting Information: Files S1-S6, Figures S1-S2, and Tables S1-S4 (PDF, 1 MB)
  • File S1: Assessing the inter-chromosomal pattern when centromere locations are unknown. (PDF, 86 KB)
  • File S2: Expected offspring heterozygosity under central vs. terminal fusion. (PDF, 219 KB)
  • File S3: Detailed RAD-sequencing protocol and analysis of RAD-sequencing data. (PDF, 95 KB)
  • File S4: Raw and corrected SNP data set for all offspring of the AST-01-04 NMP clone. (.xlsx, 337 KB)
  • File S5: Raw and corrected SNP data set for all offspring of the RM-1-18 MP clone. (.xlsx, 522 KB)
  • File S6: The genetic map v4.0.1. (.xlsx, 202 KB)
  • Figure S1: Heterozygosity depending on marker position along each linkage group. (PDF, 146 KB)
  • Figure S2: Mapping functions under different degrees of interference. (PDF, 319 KB)
  • Table S1: Microsatellite genotypes of parents and offspring. (PDF, 295 KB)
  • Table S2: Heterozygosity of microsatellite loci in relation to the distance from the centromere. (PDF, 300 KB)
  • Table S3: Number of RAD loci and average heterozygosity per linkage group. (PDF, 300 KB)
  • Table S4: The ten most frequently observed segregation patterns. (PDF, 305 KB)