A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes

Supplemental Materials

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  • Supplemental Materials
  • Movie 1 - Video S1 Three-color live cell imaging of cells expressing GalT-iRFP670, GFP-Golgin-84, and mCherry-GM130. HeLa cells transiently expressing GalT-iRFP670, GFP-Golgin84 and mCherry-GM130 were treated with nocodazole and imaged live for 24 min with a time interval of 30 sec. The time lapse corresponds to Figure 5. The time stamp represents min:sec.