Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis

Supplemental Materials

This article contains the following supporting material:

  • Supplemental Materials
  • Movie 4 - Movie S4: Loss of Gef1 delays onset of ring constriction. Cdc15-GFP shows the cytokinetic ring in the cell division site in upper panel. Sad1-mCherry as SPB marker is shown in the bottom panel. Left panel shows a gef1+ cell, right panel shows a gef1Δ cell. Both gef1+ and gef1Δ movies start from initial SPB separation. Frame interval is 2 minute. Frame rate is 2 fps.
  • Movie 3 - Movie S3: Spatiotemporal localization of CRIB-3XGFP in scd1Δ cells during cytokinesis. Left panel shows CRIB-3XGFP. Right panel shows cytokinetic ring marker Rlc1-Tomato and SPB marker Sad1-mCherry.Frame interval is 2 minute. Frame rate is 2 fps.
  • Movie 2 - Movie S2: Spatiotemporal localization of CRIB-3XGFP in gef1Δ cells during cytokinesis. Left panel shows CRIB-3XGFP. Right panel shows cytokinetic ring marker Rlc1-Tomato and SPB marker Sad1-mCherry.Frame interval is 2 minute. Frame rate is 2 fps.
  • Movie 1 - Movie S1: Spatiotemporal localization of CRIB-3XGFP during cytokinesis. Left panel shows CRIB-3XGFP. Right panel shows cytokinetic ring marker Rlc1-Tomato and SPB marker Sad1-mCherry. Frame interval is 2 minute. Frame rate is 2 fps.
  • Movie 5 - Movie S5: Bgs1 recruitment to the division site is delayed in gef1Δ cells. Bgs1-GFP at the cell division site is shown in upper panel. Bottom panel shows cytokinetic ring marker Rlc1-Tomato and SPB marker Sad1-mCherry. Left panel shows a gef1+ cell, right panel is a gef1Δ cell. Both gef1+ and gef1Δ movies start playing from separation of SPBs. Sad1-mCherry as SPB marker was used. Frame interval is 2 minute. Frame rate is 2 fps.
  • Movie 6 - Movie S6: Loss of Scd1 delays ring constriction but not onset of ring constriction. Rlc1-Tomato shows the cytokinetic ring in the cell division site. Sad1-mCherry represents SPBs separation. Left panels show bright field images. Right panels shows both Rlc1-Tomato and Sad1-mCherry. Upper panel shows a scd1+ cell, bottom panel shows a scd1Δ cell. Both scd1+ and scd1Δ movies start playing from separation of SPBs. Frame interval is 2 minute. Frame rate is 2 fps.