Construction route of the recombinant vector pDBN10879 (Fig. S1); SDS-PAGE analysis of the purified methyltransferases from scaffold 02 and Dmt (Fig. S2); HPLC analysis of the products during dicamba conversion by Dmt (methyltransferase from scaffold 66) (Fig. S3); DNA elements in the dmt gene cluster promoter (Fig. S4); transcriptional analysis of dmt, metF, purU, and dhc in scaffold 66 of strain Ndbn-20 grown with glucose or dicamba (Fig. S5); effect of temperature and pH on the activity of Dmt (Fig. S6); alignment of nucleotide sequences of the dmt gene and the codon-optimized dmt gene (Fig. S7).
PDF, 1.3M