A Lytic Polysaccharide Monooxygenase with Broad Xyloglucan Specificity from the Brown-Rot Fungus Gloeophyllum trabeum and Its Action on Cellulose-Xyloglucan Complexes

Supplemental material

• Supplemental file 1 -

  Splicing variants of the gene encoding GtLPMO9A (Fig. S1); sequences of the C-terminal extensions of GtLPMO9A-2 and GtLPMO9D (Fig. S2); multiple sequence alignment of the C-terminal domain of GtLPMO9A­-2 with homologous domains (Fig. S3); multiple sequence alignment of the C terminus of GtLPMO9D with similar C termini of LPMOs (Fig. S4); SDS-PAGE of recombinant GtLPMO9A-2 produced in Pichia pastoris (Fig. S5); comparison of the product profile of GtLPMO9A-2 on PASC with ascorbic acid or DTT as electron donor (Fig. S6); products generated by GtLPMO9A-2 or NcLPMO9C from oligosaccharides (Fig. S7); products generated by GtLPMO9A-2 or NcLPMO9C from carboxymethylcellulose (Fig. S8); HPAEC-­PAD analysis of reaction products generated from konjac glucomannan by GtLPMO9A-2 or NcLPMO9C in the dynamic viscosity experiments (Fig. S9); details of the Hex₆Pen₃ and Hex₇Pen₅/Hex₈Pen₄ ion clusters in the MALDI-­ToF spectrum for the end-­point sample from the dynamic viscosity analysis where GtLPMO9A-2 or NcLPMO9C reacted with tamarind xyloglucan in the presence of reducing agent (Fig. S10); HPAEC-PAD analysis of reaction products generated by GtLPMO9A-2 or NcLPMO9C on glucomannan-coated PASC (Fig. S11).

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