Primer name

Primer sequence

BamHI-flaA-595

CGGGATCCCGCACAAGTAAGTAAGCCGAATATTCATC

flaA-ext

CTTGCGCTTGAGTCATGCCTTCGTTATTTTTACGAAG

5-Bam-withP-lmo0674

CGGGATCCCACCTTTCACTCCCTTCATCAGATTAC

3-Sal-lmo0674

ACGCGTCGACGAATAGATTCACCATTTACGGTATAAATTTCG

int-seq-lmo0674

CAAGTAGAAGATATTCCGAAAAACAAG

5' SacI DraII rrnB term

CCTGAGCTCGGGCCCTTTCGTCTTCAAGAATTAA

3' SacI rrnB term

CGGGAGCTCGAATTCCGATCCCCAATTCC

5' SacI Phyperspac (-40-+1) EagI

P- CAATTTTGCAAAAAGTTGTTGACTTTATCTACAAGGTGTGGCATAATGTGTGTC

3' SacI Phyperspac (-40-+1) EagI

P- GGCCGACACACATTATGCCACACCTTGTAGATAAAGTCAACAACTTTTTGCAAAATTGAGCT

5' ermC incl -35

CCCAAAGTGCACAATCGCATCCGATTGCAG

3' PvuI ermC

GGGTTTCGATCGGGCACACGAAAAACAAGT

5-Bam-flaA-full-P

CGGGATCCGAAGTGTTTGGACAACTTTTCTGTTCAA

BamHI-flaA-724

CGGGATCCTAAAAACATAAAAACGTTGATATAAAGCCG

BamHI-flaA-780

CGGGATCCCAACTTATTTTTACATTTTTAATAAAAAACATTTG

5-Bam-flaA-35

CGGGATCCAACATTTGATTTTTTAAAAAAATGAAGATA

5-Bam-flaA-NO-P

CGGGATCCAACCGAATGTAGGAGGGAAACACAAATG

3-KpnI-LacZ

GGGGTACCTTATTTTTGACACCAGACCAACTGG

5-1kb-Kpn-lmo0674

GGGGTACCGTCCAACAAGCACTTGGTTGGGCGGCAAATTCG

5-int-lmo0674

TTCTTTGAATACAAGTAATTTTCTTATTTCTGATTTAGGCAT

3-1kb-Bam-lmo0674

CGGGATCCGTAGAGTTTAACTAAAATACCTCAATAACATTTTG

3-int-lmo0674

AGAAAATTACTTGTATTCAAAGAAAATTATAAACAAATGTAA

cheY-ext

ATCTTTCACGATATTCTTAATCATCGTACGCATGAACATCGC

lmo0675-ext

ACTAAAAAATAAGAAATTCCCGCAAGTACTGCCAAAAGTAC

327-biotin

B- CGCACAAGTAAGTAAGCCGAATATTCATC

3' EcoRV shift with P

ACGCGATATCCTTCTCTATATGCTTTATTATATCTTC