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Kakoki et al. 10.1073/pnas.0405449101. |
Table 3. Primers for amplification and probes for detection by quantitative RT-PCR
|
mRNA |
Primer/probe |
Sequence |
|
B2 receptor |
Forward primer |
5'-ATCTTGCAGGTGCTGAGGAA-3' |
|
B2 receptor |
Reverse primer |
5'-AGCACAAAGAGCCCCAGGA-3' |
|
B2 receptor |
Probe |
5'-FAM-CAGCACTAGCACGGTGGCCTTCCT-Tamra-3' |
|
B1 receptor |
Forward primer |
5'-GTTTCAACTGGCCCTTTGGA-3' |
|
B1 receptor |
Reverse primer |
5'-CACCAGGAAGATGCTGATGA-3' |
|
B1 receptor |
Probe |
5'-FAM-CCTCTGCCGGGTGGTCAGCG-Tamra-3' |
|
Megsin |
Forward primer |
5'-GGCGGTTCAATTTGTCTACCA-3' |
|
Megsin |
Reverse primer |
5'-ATGTACATGCTTATGCCACCA-3' |
|
Megsin |
Probe |
5'-FAM-TCAGCAGCCACCAATGCAGGTTCTT-Tamra-3' |
|
Nephrin |
Forward primer |
5'-CAGCTGCTAGTCTGCGAGG-3' |
|
Nephrin |
Reverse primer |
5'-ATCAATGACAGGAGGTCCTG-3' |
|
Nephrin |
Probe |
5'-FAM-TCCAACCCAGCCTTGGCCACTC-Tamra-3 |