A cationic lipid-formulated plasmid DNA vaccine confers sustained antibody-mediated protection against aerosolized anthrax spores -- Data Supplement - HTML Page - 05557Table3.htslp -- Proceedings of the National Academy of Sciences

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Table 3. Summary of results from in vitro expression and mouse in vivo immunogenicity studies

Antigen encoded	Description of encoded antigen	In vitro expression	Mouse immunogenicity	Letx neutralization

h-PA63	Truncated PA with human optimized codons	++++	+++	+++
h-PA63	h-PA63 with mutated Phe313/Phe314	++++	+++	+++
h-PA83	Full-length PA with optimized codons and a mutated furin cleavage site (RKKR)	+++	++++	++++

Ba-PA63	Truncated PA with B. anthracis codons	+++	+++	++
Ba-PA83	Full-length PA with B. anthracis codons	++	++++	+++

h-LF[I-IV]	Full-length LF with optimized codons and a mutated metalloprotease active site, HEXXH	++	++	+

h-LF[I]	Codon optimized LF domain I alone	++++	++++	+
h-LF[I-III]	Codon optimized LF domains I-III	+++	++++	+
Ba-LF-E687C	Full-length LF with B.anthracis codons and a mutated metalloprotease active site, HEXXH	++	++	+

Ba-LF4	LF domain I alone with B. anthracis codon	++++	++++	+
h-PA63	h-PA63 with all 10 N-linked glycosylation sites mutated	+	-	-
h-LF93	h-LF[I-IV] with all 7 N-linked glycosylation sites mutated	+	-	-

In vitro expression was measured by Western blot from supernatants and lysates of a transfected mouse melanoma cell line. Mouse immunogenicity studies measured the anti-PA or anti-LF IgG titers and Letx neutralization titers in immunized mouse serum exactly as described in Materials and Methods. A representative mouse immunogenicity study comparing humanized and B. anthracis codon plasmids for PA is shown in Fig. 5. The number of plus symbols is an indication of the relative level of in vitro expression and in vivo immunogenicity. A minus symbol indicates the lack of measurable immunogenicity.