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Table 4. Oligonucleotide primers used in this study
|
ORF designation* |
Primer sequence† (5' to 3') |
Location* |
|
BBA68 |
F-AGTTTTAATTTATAAGGAGAG |
lp54 |
|
|
R-TTTCTATTAGTAAAAGGCAGG |
|
|
BBA69 |
F-GGTTTTAATTTATAAGGAGAG |
lp54 |
|
|
R-AATAAAAGGCAGATTGTAAAG |
|
|
BBA70 |
F-CCGTTAGTCCAATGGCTCCAG |
lp54 |
|
|
R-ATGCAAATTAAAGTTAATATC |
|
|
BBC02 |
F-TTTGCACATATTATTGCG |
cp9 |
|
|
R-CAACATAAGTATCTATCT |
|
|
BBC06 |
F-GATGGTAGATAATAACAA |
cp9 |
|
|
R-AACTTAATCTTTAGGCAA |
|
|
BBC07 |
F-GATGGTAGATAATAACAA |
cp9 |
|
|
R-AACTTAATCTTTAGGCAA |
|
|
BBD14 |
F-ATGATAATAAAAATAAAAAATAATG |
lp17 |
|
|
R-ATTTTGATTAATTTTAATTTTGCTG |
|
|
BBE17 |
F-GCGGCGATGAAAGTAATAATA |
lp25 |
|
|
R-GAAAAATCATATCAAATGCAC |
|
|
BBG22 |
F-ATGATAACACAASAAGACTACAC† |
lp28-2 |
|
|
R-AAGATCGTATCTGTAAAAAGA |
|
|
BBG23 |
F-ATGWTAAGTGATGATGATTTTG |
lp28-2 |
|
|
R-CCAATCATATCTGTAGAATGA |
|
|
BBG32 |
F-ATGAAAGTCGCYAGTCTTATAAGG |
lp28-2 |
|
|
R-ATTCGAAAATTTTATRGTTTTTGG |
|
|
BBI38 |
F-GGGCGTTGAAAAACTTTAAAT |
lp28-4 |
|
|
R-TAGTAGATAAAGAGGATGGG |
|
|
BBJ18 |
F-ASAWATAGCCAACAACAT |
lp38 |
|
|
R-TGCTCTTGCCTACACCTC |
|
|
BBJ19 |
F-ATGGCATTAAATTTGCTTAACC |
lp38 |
|
|
R-TTATCTATTTCTTCAATTCTA |
|
|
BB0082 |
F-TCTATTACAAAGACCACTGCTAG |
Chromosome |
|
|
R-TTTAATCATCACATTATAAATTTTA |
|
|
BBQ45 |
F-TAAAGTAACTTCAAAGCG |
lp56 |
|
|
R-CAAGAACTTATCAGCACA |
|
|
BBQ47 |
F-SYATTTGCAATGGAGAGATT |
lp56 |
|
|
R-AGAGAGTATATAAATAAACAA |
|
|
BBQ48 |
F-RMAATTAAAGGATACATC |
lp56 |
|
|
R-CACTAATTGTTACTCTAG |
|
|
BBQ49 |
F-GAATGCTAGAAAAATGTT |
lp56 |
|
|
R-TTGTGATATATCAACTCC |
|
*B. burgdorferi strain B31 ORF and plasmid names.
†
Codes for nonstandard bases are as follows: M = A + C, R = A +G, S = C + G, W = A + T, Y = C + T.