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Luzzi et al. 10.1073/pnas.0406216101. |
Table 1. Q-PCR primer and fluorescence resonance energy transfer (FRET) probe list
|
Murine mRNA, (GenBank accession no.) |
Forward primer |
Reverse primer |
FRET probe |
|
ZnT1 (NM_009579) |
CACGACTTACCCATTGCTCAAG |
CTTTCACCAAGTGTTTGATATCGATT |
AGTCTGCTCATTCTTCTACAAACTGTCCCTAAGC |
|
ZnT2 (NM_131731) |
CCGACCAGCCACCAAGAC |
TGGAAAGCACGGACAACAAG |
CGGCTCGAGCCAGCCGAA |
|
ZnT4 (NM_011774) |
GCTGAAGCAGAGGAAGGTGAA |
TCTCCGATCATGAAAAGCAAGTAG |
CAGGCTGACCATCGCTGCCGT |
|
ZnT5 (NM_022885) |
CTGCTCGGCTTTGGTCATG |
CGGCCATACCCATAGGAAGAA |
TTTGCTGCCCTGATGAGCCGC |
|
ZnT6 (NM_144798) |
TCCCAGGACTCAGCAGTATCTTC |
GCCCCAGCAAGATCAATCAG |
TGCCCCGCATGAATCCGTTTG |
|
ZnT7 (NM_023214) |
CCTCTCTTTCGCTTTTGTGGAA |
GTGGAAGGAGTCGGAGATCAAG |
ACTCTACGGCATCTGGAGCAACTGCCT |
|
ZnT8 (NM_172816) |
TGGGTGGTATCGAGCAGAGAT |
ACACCAGTCACCACCCAGATG |
TCGGTGCCCTGCTGTCTGTCCTT |
|
ZIP1 (NM_013901) |
TCCTCAAGGTCATTCTGCTCCTA |
CCCTTTCTCTTGAAGCACCTTAGA |
CTGCTCACTGGCCTTCTCTTTGTCCAA |
|
ZIP2 (NM_139051) |
CTGCTTGCTCTTCTGCTTCTCA |
GACCTGTAGCTGCATCCATCTG |
ACTGGGCTGTGGCCTTACTCCCATCTAC |
|
ZIP3 (NM_134135) |
CTGGGCTACGCCGTTCTG |
GGGACGTGCTCTGTGTCCTT |
CTTTCTCAAGTGGTGAGCCCTGAATCCC |
|
ZIP4 (NM_028064) |
CTCTGCAGCTGGCACCAA |
CACCAAGTCTGAACGAGAGCTTT |
CAATCTCCGACAGTCCAAACAGACCCAT |
|
ZIP5 (NM_028051) (NM_028092) |
GGGCAGCCTCATGTTTACCA |
CCACATCAGCCGTCAGGAA |
CCCTATTGGAGGAGCAGCTAGTGCCC |
|
ZIP6 (LIV-1) (NM_139143) |
GCCACAGCCAGCGCTACT |
ATCACCATCCAGGCCAATGT |
CGGCGTCCTTCAGCTCCTCTCTCGA |
|
ZIP7 (KE4) (NM_008202) |
AGGCATCAAACACCACCTGG |
TGCGGAGATCAGCACTGTG |
CTGTCACCCTCTGGGCCTACGCACT |
|
ZIP8 (mBIGM103) (NM_026228) |
CTAACGGACACATCCACTTCGA |
CCCTTCAGACAGGTACATGAGCTT |
ACTGTCAGCGTTGTATCCCTCCAGGATG |
|
ZIP14 (NM_144808) |
GAGCCAACTGATAATCCATTGCT |
GTCAACGGCCACATTTTCAA |
TTGGCAACAGATTCGCCCCATGA |
FRET probes are 5′ FAM (6-carboxyfluorescein) and 3′ BHQ1 (2,5-di-tert-butylhydroquinone-1).