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Fig. 7. A 2.5-Å F_o – F_c electron density map contoured at 3s around the inhibitor, which was omitted during calculation. The inhibitor is drawn in stick representation. Red, oxygen; blue, nitrogen; orange, sulfur; green, carbon. Zn²⁺ and K⁺ ions are indicated.

Fig. 8. Thermal unfolding of HDAC8 monitored by CD spectroscopy at 222 nm. The gray and black curves represent the unfolding transition in the presence of 15 mM NaCl or 15 mM KCl, respectively. (Inset) Far-UV CD spectra of HDAC8 in 15 mM NaCl (gray line) or 15 KCl (black dashed line) before unfolding.