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Fig. 5. The linear force-velocity relationship assumed for KLP61F motors.

Fig. 6. Function depicting the relation between the average number of active KLP10A polymerases per minus end and the mean depolymerization rate of the minus end. r_c is the critical value of the average number of active KLP10A polymerases per minus end at which the mean depolymerization rate starts to deviate from zero, and r_s is the saturation level of this number beyond which no change in the depolymerization rate is observed.

Fig. 7. Numerical solutions of the poleward flux and spindle elongation rates in KLP3A inhibited spindles. The best fit to experimental data for KLP3A inhibited embryos (blue) is obtained when the number of interpolar microtubule (ipMT) arrays, N, is varied between 5 and 14 corresponding to high and low mean flux rates (orange). For each value of N, the corresponding depolymerization rate is calculated using the function in Fig. 6, assuming N = 40 for control embryo. The model solutions (orange) are superimposed on the data from Fig. 1C for KLP3A inhibited (blue) and control (gray) embryos. All other parameters are as in Table 2.

Movie 1. The dynamics of realistic ipMTs in a control metaphase/anaphase A or a KLP3A-inhibited anaphase B spindle at isometric length; parameters are as in Fig. 4B.

Movie 2. The dynamics of realistic ipMTs in an anaphase B spindle that is elongating; parameters are as in Fig. 4B.

Movie 3. The computed movement of fluorescent speckles in the spindle in Movie 1. Note that ipMTs slide apart persistently throughout, but during metaphase/anaphase A, the spindle does not elongate due to depolymerization at the poles, which converts ipMT sliding to flux.

Movie 4. The computed movement of fluorescent speckles in the spindle in Movie 2. Note that ipMTs slide apart persistently throughout, during anaphase B depolymerization at the poles stops, speckles move away from the equator at the same rates as the poles and the spindle elongates.

Movie 5. A computer simulation of fluorescence recovery after photobleaching (FRAP) in the bleached zone of the central anaphase B spindle (recovery kinetics shown in Fig. 4A).

Movie 6. The dynamics of ipMTs and the poles in the spindle matrix model for anaphase B. The yellow lines in the central spindle region represent an 8 mm long spindle matrix against which KLP61F motors slide the MTs. The spindle elongates steadily despite the highly dynamic central region.

Movie 7. The dynamics of ipMTs and the poles in the astral pulling model for anaphase B. The green circles denote the position of the left and right spindle pole, which are being separated by dynein pulling the astral MTs (red arrowheads). The spindle elongates steadily as the poles are being separated, and the ipMTs are sliding apart freely by KLP61F motors, toward the moving poles.