Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - AtRAB8 and the three BTI proteins do not interact with VirB1, VirB1*, VirB5, VirD2, VirE1, VirE2, or VirF in yeast. Virulence proteins were tested for interaction with BTI proteins or AtRAB8 using a two-hybrid system as described in Methods.
• Supplemental Figure 2 - DNA sequence alignment of the three BTI cDNA sequences based on the CLUSTAL W program (Thompson et al., 1994). * indicates identical nucleotide sequences.
• Supplemental Figure 3 - DNA sequence alignment of the three BTI genomic DNA sequences based on the CLUSTAL W program (Thompson et al., 1994). * indicates identical nucleotide sequences.
• Supplemental Figure 4 - BTI1 A/S and RNAi transgenic plants have reduced levels of BTI1 protein. (A) Western blot analysis of BTI1 in transgenic A/S, RNAi, and wild-type plants. (B) Western blot analysis of actin in transgenic A/S, RNAi, and wild-type plants. (C) Protein levels of BTI1 in transgenic A/S and RNAi plants are shown as a relative percentage of that in wild-type plants. BTI1 protein amounts were normalized based on actin protein amounts in the same protein sample. The ratio of BTI1 protein/actin protein was set as 100% in the wild-type plant. Data are shown as average values of three Western blot analyses from three T2 generation plants of each line. Error bars indicate standard error.
• Supplemental Figure 5 - Western blot analysis of BTI1 protein from Arabidopsis suspension cell cultures following infection by A. tumefaciens At849. (A1) Equal quantities of protein from Arabidopsis suspension cells incubated without Agrobacterium were loaded onto an SDS-polyacrylamide gel. Following electrophoresis, the gel was stained with Coomassie Blue. Numbers above the lanes indicate hours post-infection. (A2) Western blot analysis using anti-BTI1 antibodies. (A3) Western blot analysis using anti-actin antibodies. (B1) Equal quantities of protein from Arabidopsis suspension cells infected with A. tumefaciens At849 were loaded onto an SDS-polyacrylamide gel. Following electrophoresis, the gel was stained with Coomassie Blue. (B2) Western blot analysis using anti-BTI1 antibodies. (B3) Western blot analysis using anti-actin antibodies. Mw, Molecular weight markers.
• Supplemental Table 1
• Supplemental Table 2
• Supplemental Table 3