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Fig. 7. Cytotoxicity measurements of poly(b -amino esters). COS-7 cells were incubated with polymer in Opti-MEM for 1 h, and metabolic activity was measured 24 h later. Measurements were performed in quadruplicate, and SDs are shown for C32 and polyethyleneimine (PEI).

Fig. 8. Inhibition of luciferase activity by C32-delivered DNA encoding the A chain of diphtheria toxin (DT-A). LNCaP cells were incubated with C32/DNA complexes for 1 h, after which the medium was changed. Forty-eight hours later, cells were harvested and protein extracts were prepared and assayed for luciferase activity. The DNA constructs used are indicated below each bar. luc, C32-pCAG/luc; enhanced green fluorescent protein (EGFP), C32-pRSV/FRT2PSA.FLP/EGFP; DT-A, C32-pRSV/FRT2PSA.FLP/DT-A. The experiment was repeated three times.