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Fig. 5. Wild-type embryos at stage 15/16 injected with injection buffer (A, C, E, and G) or double-stranded RNAs (dsRNAs) (B, D, F, and H). Embryos were stained with anti-FasII (A and B), anti-prospero (C and D), anti-engrailed (E and F), or anti-elav (G and H) Abs. Note that injection of each dsRNA reduced the level of the corresponding endogenous protein significantly.

Fig. 6. Known phenotypes of signaling molecules. Stage 15/16 embryos were stained with BP102 (F, G, and J) 22C10 (A–D, H, and I) or 1D4 (E) mAbs after injection of the dsRNA indicated at top of each image. Ventral views of embryos are shown in C, E–G, and J) and lateral views (A, B, D, H, and I).

Fig. 7. Known phenotypes of transcription/RNA processing factors. Stage 15/16 embryos were stained with BP102 (H) or 22C10 (A–G) mAbs after injection of the dsRNA indicated at top of each panel. Note hypoplasia of neurons in A, instead of hyperplasia characteristic of neurogenic genes in the Notch pathway. Ventral views are shown in A and F–H and lateral views are shown in B–E.

Fig. 8. Additional known phenotypes. Stage 15/16 embryos were stained with 22C10 mAb after injection of the dsRNA indicated at top of each image. Ventral views are shown in A and E and lateral views in B–D and F.