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Martines-Argudo et al. 10.1073/pnas.0405312101.

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Supporting Figure 6
Supporting Table 3
Supporting Figure 7




Supporting Figure 6

Fig. 6. Nitrogen-fixation phenotype of Azotobacter vinelandii transformants. A. vinelandii strains were streaked onto NIL agar medium (containing 20 g/liter sucrose, 0.2 g/liter MgCl2, 0.09 g/liter CaCl2, 0.8 g/liter KH2PO4, 0.2 g/liter K2HPO4, 0.12 g/liter Fe2 (SO4)3, and 2.4 mg/liter Na2MoO4) either with added nitrogen (25 mM ammonium acetate, +N) (A) or without added nitrogen (–N) (B) and incubated at 30°C for 2–3 days. 1, Wild-type A. vinelandii; 2, mutant nifL-R306C; and 3,double mutant nifL-R306C, nifA-E356K.





Supporting Figure 7

Fig. 7. Influence of substitutions of NifL arginine 306 on NifA activity in vivo. Cultures were grown and assayed for b -galactosidase activity from the nifH::lacZ reporter, as described in Table 1. Cross-hatched bars indicate cultures grown anaerobically under nitrogen-limiting conditions (–O, –N), dark-gray bars indicate growth under anaerobic nitrogen-excess conditions (–O, +N), light-gray bars indicate cultures grown aerobically under nitrogen-limiting conditions (+O, –N), and black bars indicate cultures grown arerobically under nitrogen-excess conditions (+O +N). Western blot analysis of NifL protein accumulated in cultures used for b-galactosidase assays is shown below the graph.