Botrytis fragariae, a New Species Causing Gray Mold on Strawberries, Shows High Frequencies of Specific and Efflux-Based Fungicide Resistance

Supplemental material

  • Supplemental file 1 -

    PCR products of B. cinerea strain B05.10, B. fragariae strains D13_F_Me3, D11_H_R4, and D14_F_Ju20, and B. pseudocinerea strain VD110 with primer pair Mrr1-spez-F/Mrr1-spez-R (Fig. S1); phylogenetic trees of Botrytis spp., including four strains of B. fragariae from Germany (strains D11_H_R4, D13_F_Me3, and D13_D_F_Ju10) and South Carolina (strain U14_H3), based on combined sequences of hsp60, g3pdh, rpb2, nep1, and nep2 (Fig. S2); identification of B. fragariae by PCR-RFLP (Fig. S3); frequency of occurrence of B. fragariae, B. cinerea, and B. pseudocinerea in Germany (Fig. S4); phylogenetic tree of four German and three U.S.-American B. fragariae strains, based on hsp60, g3pdh, rpb2, nep1, and nep2, using B. cinerea as the outgroup (Fig. S5); infection of strawberry tissues by B. fragariae and B. cinerea (Fig. S6); distribution of fungicide resistance in 38 B. fragariae isolates (Fig. S7); primers used in this study (Table S1); accession numbers of sequences used for assembling the phylogenetic trees (Table S2); occurrence of Botrytis species on wild strawberries (Fragaria vesca) in western Germany (Table S3); fungicide treatments in fields from which B. fragariae isolates were recovered (Table S4); Botrytis strains used for quantitative fungicide sensitivity assays and atrB expression studies (Table S5).

    PDF, 962K