Molecular determinants of the N-terminal acetyltransferase Naa60 anchoring to the Golgi membrane

Supplemental Data

• Table S1 and Figures S1-S4 (.pdf, 2.4 MB) - Table S1. All mutations performed on Naa60, purpose of the mutation and consequence observed by microscopy and carbonate wash assay when applied. Figure S1. Mutated constructs of Naa60-eGFP demonstrated hydrophobic amino acids within Pred-?????????????????1 important for membrane association in cellulo. Figure S2. Mutated constructs of Naa60-eGFP demonstrated amino acids within Pred-?????????????????2 important for membrane association in cellulo. Figure S3. Anti-Naa60-192-242 antibody validation for detection of Naa60-189-242 peptide. Figure S4. Far UV-CD experiments supporting Naa60189-242 folding most optimal in the presence of PI4P over all other PI.