The Legionella pneumophila Incomplete Phosphotransferase System Is Required for Optimal Intracellular Growth and Maximal Expression of PmrA-Regulated Effectors

Supplemental material

• Supplemental file 1 -

  Fig. S1. Sequence alignment of the NPr protein from different Legionella species. Fig. S2. In vitro growth profiles of the ΔptsP, ΔptsO, and ΔptsP-ptsO mutants. Fig. S3. Effect of the L. pneumophila PmrAB TCS on its target effector-encoding genes. Fig. S4. PmrA must be phosphorylated in order to activate gene expression.

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• Supplemental file 2 -

  Data Set S1. Plasmids used in this study.

  XLS, 48K

• Supplemental file 3 -

  Data Set S2. Primers used in this study.

  XLS, 41K