Specific growth rates of engineered strains of T. kodakarensis in ASW-YT-Pyr medium (Table S1); construction of a ChiA overexpression strain of T. kodakarensis (Fig. S1); construction of the chiA overexpression vector pUD3-Pcsg-chiA (Fig. S2); construction of the dac and chiA overexpression vector pUD3-dac-Pef-1α-Pcsg-chiA (Fig. S3); construction of the plasmid pUD3-Pgdh-dppA for overexpression of ABC transporter genes glmD and glmA (Fig. S4); construction of the plasmid pLC-tgr for complementation of tgr (Fig. S5); comparison of sequences in Pgdh regions of KC04Δt and KC04ΔtM1 (Fig. S6); growth curves of T. kodakarensis strains in ASW-YT-Pyr medium (Fig. S7); effect of tgr gene reintroduction on chitin-dependent H2 production in KC04ΔtM1 (Fig. S8); expression levels of GK, PFK, and Tgr detected by Western blot analyses (Fig. S9 and S10).
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